A Cross Sectional Study of Prevalence of Various Dengue Serotypes Along with Hematological and Biochemical Profiles of Dengue Patients in Western Part of India
Authors/Creators
- 1. Assistant Professor, Department of Pathology, Namo Meri, Silvassa, DNH
- 2. Assistant Professor, Department of Microbiology, Namo Meri, Silvassa, DNH
- 3. Assistant Professor, Department of Community Medicine, Zydus Medical College and Hospital, Dahod, Gujarat
- 4. Associate Professor, Department of Microbiology, Namo Meri, Silvassa, DNH
- 5. Tutor Cum Statistician, Department of Community Medicine, Zydus Medical College and Hospital, Dahod, Gujarat
Description
Introduction: As per WHO, the 30 most highly endemic countries for Dengue in the world includes five countries as India, Indonesia, Myanmar, Sri Lanka and Thailand (December 2020). In India total 10172 cases were reported and 3 deaths were reported till 22 June 2022. In Dadara Nagar haveli, total 547 and 46 cases were reported during 2021 and till 22 June 2022 respectively. Objective: The objectives of this study are1) to find out prevalence of various Dengue serotypes of the given population, 2) to compare hematological and biochemical parameters of the various dengue serotypes. Methods: A hospital based cross sectional study was conducted in a tertiary care hospital at western part of India during January 2021 to December 2021.Those study participants who fulfilled the inclusion criteria were enrolled in the study. Hematological and biochemical parameters tests were done. The statistical ANOVA test is applied to check significance of various parameters among serotypes. Results: During the study period, a total of 100 dengue cases were included in the study. Mean age±SD of dengue patients was 27.15±10.82 years along with this 89% were in the age group of 11 to 40 years. Males were found to be 67 % and females were 33%. Maximum proportion of the patients belonged to DEN 2 serotyping, which is 53%, followed by DEN1 serotyping, i.e., 23 %. Conclusion: The study revealed that, dengue positivity was higher in male population than in females, DEN2 serotype was higher as compared to other serotype of dengue virus viz. DEN1, DEN3 and DEN4 as well as some of the patients were co-infection with serotypes-1 and 3 and serotypes-2 and 3. In dengue patient’s hematological parameters like, total leucocyte counts, and PCV/Hematocrit was increased while platelet counts decreased. Biochemical parameters like, ALT, AST, Serum Bilirubin Total, have found to be higher than normal range. Dengue Type 2 serotype is more severely causing reduction in platelet, leucocytes, RBC counts, and HB level as compared to other serotypes. This can be correlated with its antigenic structure that have more virulence power as compared to other serotype.
Abstract (English)
Introduction: As per WHO, the 30 most highly endemic countries for Dengue in the world includes five countries as India, Indonesia, Myanmar, Sri Lanka and Thailand (December 2020). In India total 10172 cases were reported and 3 deaths were reported till 22 June 2022. In Dadara Nagar haveli, total 547 and 46 cases were reported during 2021 and till 22 June 2022 respectively. Objective: The objectives of this study are1) to find out prevalence of various Dengue serotypes of the given population, 2) to compare hematological and biochemical parameters of the various dengue serotypes. Methods: A hospital based cross sectional study was conducted in a tertiary care hospital at western part of India during January 2021 to December 2021.Those study participants who fulfilled the inclusion criteria were enrolled in the study. Hematological and biochemical parameters tests were done. The statistical ANOVA test is applied to check significance of various parameters among serotypes. Results: During the study period, a total of 100 dengue cases were included in the study. Mean age±SD of dengue patients was 27.15±10.82 years along with this 89% were in the age group of 11 to 40 years. Males were found to be 67 % and females were 33%. Maximum proportion of the patients belonged to DEN 2 serotyping, which is 53%, followed by DEN1 serotyping, i.e., 23 %. Conclusion: The study revealed that, dengue positivity was higher in male population than in females, DEN2 serotype was higher as compared to other serotype of dengue virus viz. DEN1, DEN3 and DEN4 as well as some of the patients were co-infection with serotypes-1 and 3 and serotypes-2 and 3. In dengue patient’s hematological parameters like, total leucocyte counts, and PCV/Hematocrit was increased while platelet counts decreased. Biochemical parameters like, ALT, AST, Serum Bilirubin Total, have found to be higher than normal range. Dengue Type 2 serotype is more severely causing reduction in platelet, leucocytes, RBC counts, and HB level as compared to other serotypes. This can be correlated with its antigenic structure that have more virulence power as compared to other serotype.
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IJPCR,Vol14,Issue7,Article14.pdf
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Additional details
Dates
- Accepted
-
2022-06-05
Software
- Repository URL
- https://impactfactor.org/PDF/IJPCR/14/IJPCR,Vol14,Issue7,Article14.pdf
- Development Status
- Active
References
- 1. Anderson CR, Downs WG, Hill AE. Isolation of dengue virus from a human being in Trinidad. Science. 1956;124(3214):224-5. 2. Chambers TJ, Hahn CS, Galler R, Rice CM. Flavivirus genome organization, expression, and replication. Annu Rev Microbiol.1990;44:649-88. 3. Brasil. Ministério da Saúde. Secretaria de Vigilânciaem Saúde. Dengue: diagnóstico e manejoclínico. 2nd ed. Brasília, DF; 2005. [cited2010 Nov 20]. Available from: http://portal.saude.gov.br/portal/arquiv os/pdf/dengue_manejo_clinico_2006.p dfx5. 4. Data obtained from National vector borne disease control program, Ministry of Health and family welfare, Government of India available at https://nvbdcp.gov.in/index4.php?lang =1&level=0&linkid=431&lid=3715. 5. https://www.who.int/news-room/factsheets/detail/dengue-and-severedengue#:~:text=The%20number%20of %20dengue%20cases,affecting%20mo stly%20younger%20age%20group. 6. Data from National vector borne disease control program, available at https://nvbdcp.gov.in/index4.php?lang =1&level=0&linkid=431&lid=3715 7. Narayanan M, Aravind MA, Thilothammal N, Prema R, Sargunam CS, Ramamurty N: Dengue fever epidemicin Chennai--a study of clinical profile and outcome. Indian Pediatr. 2002, 39:1027-1033 8. Ferede G., Tiruneh M., Abate E. et al. A study of clinical, hematological, and biochemical profiles of patients with dengue viral infections in Northwest Ethiopia: implications for patient management. BMC Infect Dis. 2018:18, 616. 9. Gupta A, Rijhwani P, Pahadia M R, et al. Prevalence of Dengue Serotypes and Its Correlation with the Laboratory Profile at a Tertiary Care Hospital in Northwestern India. Cureus May 14, 202:13(5): e15029. 10. Afreen N, Deeba F, Naqvi I, Shareef M, Ahmed A, Broor S, Parveen S: Molecular investigation of 2013dengue fever outbreak from Delhi, India. PLoS Curr. 2014,6: 10.1371/currents.outbreaks.0411252a8 b82aa933f6540abb54a855f. 11. Mishra G, Jain A, Prakash O, et al.: Molecular characterization of dengue viruses circulating during 2009-2012 in Uttar Pradesh, India. J Med Virol. 2015, 87:68-75. 12. Shastri J, Williamson M, Vaidya N, Agrawal S, Shrivastav O: Nine-year trends of dengue virus infection in Mumbai, Western India. J Lab Physicians. 2017, 9:296-302. 13. Kao CL, King CC, Chao DY, Wu HL, Chang GJ. Laboratory diagnosis of dengue virus infection: current and future perspectives in clinical diagnosis and public health. J Mibrobiol Immunol Infect. 2005; 38(1): 5-16 14. Ageep AK, Malik AA, Elkarsani MS. Clinical presentations and laboratory findings in suspected cases of dengue virus. Saudi Med J. 2006; 27(11): 1711-3 Comment in: Saudi Med J. 2007;28(8):1304; author reply 1304 15. Kuo CH, Tai DI, Chang-Chien CS, Lan CK, Chiou SS, Liaw YF. Liver biochemical tests and dengue fever. Am J Trop Med Hyg 1992; 47: 265- 270. 16. Parkash O, Almas A, Jafri SM, Hamid S, Akhtar J, Alishah H. Severity of acute hepatitis and its outcome in patients with dengue fever in a tertiary care hospital Karachi, Pakistan (South Asia). BMC Gastroenterol 2010; 10: 43. 17. Souza LJ, Alves JG, Nogueira RM, Gicovate Neto C, Bastos DA, Siqueira EW, Souto Filho JT, CezárioTde A, Soares CE, Carneiro Rda C. Aminotransferase changes and acutehepatitis in patients with dengue fever: analysis of 1,585 cases. Braz J Infect Dis 2004; 8: 156-163. 18. Saha AK, Maitra S, Hazra S Ch. Spectrum of hepatic dysfunction in 2012 dengue epidemic in Kolkata, West Bengal. Indian J Gastroenterol 2013; 32: 400-403. 19. Trung DT, Thao le TT, Hien TT, Hung NT, Vinh NN, Hien PT, Chinh NT, Simmons C, Wills B. Liver involvement associated with dengue infection in adults in Vietnam. Am J Trop Med Hyg 2010; 83: 774-780. 20. Karoli R, Fatima J, Siddiqi Z, Kazmi KI, Sultania AR. Clinical profile of dengue infection at a teaching hospital in North India. J Infect Dev Ctries 2012; 6: 551-554.