Published September 14, 2024 | Version v1
Dataset Open

Analytical ultracentrifugation datasets of NT-2Rep-CT

  • 1. ROR icon Aalto University
  • 1. ROR icon Aalto University
  • 2. ROR icon Princeton University

Description

AUC datasets for "Metastable liquid-liquid phase separation and aging lead to strong processing path dependence in mini-spidroin solutions" publication.

Dataset RunId 861 corresponds to Fig. 2a and Fig. 4a and contains information about NT-2Rep-CT sample 20 g/l concentration in 20 mM Tris pH 8.0 and 110 mM Tris pH 7.2 buffers.

Datasets RunId 989 – 1032 correspond to Fig. 3.

RunId 989, 991, 1000, 1018 and 1026 were measured using different protein concentrations: 989: 0.675, 1.25 and 2.5 g/l; 991: 10, 5 and 20 g/l; 1000: 0.1 and 2.5 g/l.

Datasets RunId 1018 – 1024 contain information about time dependence of NT-2Rep-CT sample at 0.05, 0.1 and 1 g/l concentration in 20 mM Tris pH 8.0 buffer. RunId 1018 – first experiment (0 hour), RunId 1020 – after 24 hours, RunId 1022 – after 48 hours and RunId 1024 – after 120 hours.

Datasets RunId 1026 – 1032 contain information about time dependence of NT-2Rep-CT sample at 1, 5 and 10 g/l concentration in 20 mM Tris pH 8.0 buffer. RunId 1026 – first experiment (0 hour), RunId 1028 – after 24 hours, RunId 1030 – after 48 hours and RunId 1032 – after 120 hours.

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