Figure 3. a) Median-joining network of the mtDNA control region haplotypes of E. roumanicus and E. concolor. Circle sizes are proportional to haplotype frequencies, while circle colors correspond to defined regions as shown in the legend. Each branch represents one mutational step; if more than one mutation step is present, it is denoted by the numbers. The MJ network is presented as the shortest tree, while lost connections between haplotypes after MP processing are shown with dashed lines. Black circles represent median vectors (unsampled or ancestral haplotypes). b) Bayesian phylogenetic tree showing relationships among 30 haplotypes of E. roumanicus and E. concolor. The trees were rooted using E. europaeus as an out-group. Haplotype names correspond to those given in Table 1 and Table S1.