Published July 30, 2024 | Version http://impactfactor.org/PDF/IJTPR/14/IJTPR,Vol14,Issue7,Article6.pdf
Journal article Open

Bacteriological Analysis of Blood Cultures among the Sepsis Patients from a Tertiary Health Care Setup

  • 1. Associate Professor, Department of Cardiothorasic Surgery, Rangaraya Medical College, Kakinada.
  • 2. Assistant Professor, Department of Microbiology, Rangaraya Medical College, Kakinada.
  • 3. Associate Professor, Department of Microbiology, Rangaraya Medical College, Kakinada.

Description

Introduction: Bloodstream infections (BSIs) are a leading cause of morbidity and mortality in hospitalized patients. Coagulase negative staphylococcus (CoNS) is the leading cause for the contamination of blood cultures (BCC). With this a study was conducted to find the various pathogens isolated in the blood cultures those with BSIs. Methods: It was a prospective research, conducted in the department of Microbiology, Rangaraya Medical College, Kakinada between March to May 2024. Individuals aged >18 years suspected with BSIs were included, those on antimicrobial treatment were not considered. Two blood samples were collected as per the guidelines and Brain heart infusion (BHI) biphasic media was used for culture. Blood culture, identification were carried as per the guidelines. Results: Total 74 members were included and 22 (30%) blood cultures were positive. Klebsiella (8) was the leading isolate followed by Esch.coli (5), Staph. aureus (4) Acinetobacter baumannii (3) and CoNS (2). Maximum drug resistance (DR) was observed to ampicillin and there was no Vancomycin resistance. Conclusions:  In this study, 30% blood culture were positive. GNRs are the common isolates and Klebsiella was the common isolate. No significant DR was identified. Short duration is the limitation of this research.

Abstract (English)

Introduction: Bloodstream infections (BSIs) are a leading cause of morbidity and mortality in hospitalized patients. Coagulase negative staphylococcus (CoNS) is the leading cause for the contamination of blood cultures (BCC). With this a study was conducted to find the various pathogens isolated in the blood cultures those with BSIs. Methods: It was a prospective research, conducted in the department of Microbiology, Rangaraya Medical College, Kakinada between March to May 2024. Individuals aged >18 years suspected with BSIs were included, those on antimicrobial treatment were not considered. Two blood samples were collected as per the guidelines and Brain heart infusion (BHI) biphasic media was used for culture. Blood culture, identification were carried as per the guidelines. Results: Total 74 members were included and 22 (30%) blood cultures were positive. Klebsiella (8) was the leading isolate followed by Esch.coli (5), Staph. aureus (4) Acinetobacter baumannii (3) and CoNS (2). Maximum drug resistance (DR) was observed to ampicillin and there was no Vancomycin resistance. Conclusions:  In this study, 30% blood culture were positive. GNRs are the common isolates and Klebsiella was the common isolate. No significant DR was identified. Short duration is the limitation of this research.

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Dates

Accepted
2024-06-28

References

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