Published July 17, 2024 | Version v1
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Probing the modulation of enzyme kinetics by multi-temperature, time-resolved serial crystallography

Authors/Creators

  • 1. ROR icon University Medical Center Hamburg-Eppendorf
  • 2. ROR icon Max Planck Institute for the Structure and Dynamics of Matter

Description

We present an environmental enclosure for fixed-target serial crystallography, enabling X-ray diffraction experiments in a temperature window from below 10 °C  to above 70°C - a universal parameter of protein function. Time-resolved crystallography experiments can now be carried out at physiological temperatures, providing fundamentally new insights into protein function. We show temperature-dependent modulation of turnover kinetics for the mesophilic beta-lactamase CTX-M-14 and for the hyperthermophilic enzyme xylose isomerase.

Files

20210312 SI paper HC documentation.zip

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Additional details

Dates

Created
2024-07-17