Data supplementing the article "Proficiency testing and cross-laboratory method comparison to support standardisation of diatom DNA metabarcoding for freshwater biomonitoring" submitted to "Metabarcoding and Metagenomics."

These data supplement the article "Proficiency testing and cross-laboratory method comparison to support standardisation of diatom DNA metabarcoding for freshwater biomonitoring" submitted to "Metabarcoding and Metagenomics" journal.

The directory contains the following files:

Raw_sample_fastq_files.zip - contains the raw demultiplexed fastq files (R1.fastq and R2.fastq) for each of the 275 samples used in this study to produce the ASVs table using the DADA2 bioinformatics pipeline.

DNA_Reads_Bioinformatics_Track.xlsx details the sample information used for statistical analysis (Run n°, Experiment, Participant ID,  Sample and replicate Id). Effect of bioinformatics treatments during key DADA2 bioinformatics steps on DNA reads number per sample is also provided.

ASV_table.xlsx Amplicon Sequence Variant (ASV) table with corresponding taxonomy obtained after the DADA2 bioinformatics pipeline analysis.

Molecular_Species_list.xlsx - ASV table was convert into taxonomic list at the species level. Omnidia code was provided to each diatom taxa in order to facilitate the IBD and IPS diatom indices calculation via the Omnidia 6.0 software.