Antibacterial and Phytochemical Investigation of Thuja orientalis (L.) Leaves Essential Oil from Syria
Authors/Creators
- 1. Department of Chemistry, faculty of science, Damascus University
- 2. Department of Botany, faculty of science, Damascus University
Description
Thuja Orientalis (L) (Platycladus orientalis) belongs to the family of Cupressaceae. This species is an important herb in
oriental world medicine as used in treatment of so many diseases. In this work the essential oil content variation and
antimicrobial activity have been determined. Fresh leaves of Th. Orientalis, grown in the south Syria were subjected to
hydro distillation using a Clevenger-type apparatus and the chemical composition of the essential oil have been studied
using gas chromatography-mass spectrometry (GC-MS). Mono and sesquiterpenoids in essential oil were reported as chemical constituents of this plant. Twenty-four components (97.59%) were identified. The major components were α-pinene
(22.25%), 3-carene (20.65%), cedrol (18.71%), β –Caryophyllene (6.13%), α-Humulene (5.68%), Terpinolene (4.53%),
and Limonene (3.35%). The present study led to investigate the antimicrobial activity of the essential oil against some
pathogenic microorganisms. The essential oil showed appreciable antibacterial effect against some Gram-positive less than
Gram negative bacteria. Minimum inhibitory concentration (MIC) revealed the lowest activity against Streptococcus pyogens and Vibrioparahaemplyticus (1.6 mg/mL) while the highest activity was against Bacillus subtilis and Staphylococcus
aureus (0.4 mg/mL). The MBC activity was from (1.6 mg/mL) to (3.2 mg/mL).
Abstract (English)
Thuja Orientalis (L) (Platycladus orientalis) belongs to the family of Cupressaceae. This species is an important herb in
oriental world medicine as used in treatment of so many diseases. In this work the essential oil content variation and
antimicrobial activity have been determined. Fresh leaves of Th. Orientalis, grown in the south Syria were subjected to
hydro distillation using a Clevenger-type apparatus and the chemical composition of the essential oil have been studied
using gas chromatography-mass spectrometry (GC-MS). Mono and sesquiterpenoids in essential oil were reported as chemical constituents of this plant. Twenty-four components (97.59%) were identified. The major components were α-pinene
(22.25%), 3-carene (20.65%), cedrol (18.71%), β –Caryophyllene (6.13%), α-Humulene (5.68%), Terpinolene (4.53%),
and Limonene (3.35%). The present study led to investigate the antimicrobial activity of the essential oil against some
pathogenic microorganisms. The essential oil showed appreciable antibacterial effect against some Gram-positive less than
Gram negative bacteria. Minimum inhibitory concentration (MIC) revealed the lowest activity against Streptococcus pyogens and Vibrioparahaemplyticus (1.6 mg/mL) while the highest activity was against Bacillus subtilis and Staphylococcus
aureus (0.4 mg/mL). The MBC activity was from (1.6 mg/mL) to (3.2 mg/mL).
Files
IJCPR,Vol7,Issue5,Article3.pdf
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Additional details
Dates
- Accepted
-
2016-10-01