Supplementary data for: Phylogenomics, male internal genitalia, a new species, and other notes on new world Stenopelmatus Jerusalem crickets (Orthoptera: Stenopelmatoidea: Stenopelmatini)
Authors/Creators
- 1. California Academy of Sciences
- 2. Texas A&M University
- 3. Western Ecological Research Center
Description
Based on past and expanded DNA sampling, the orthopteran families Stenopelmatidae and Anostostomatidae, as currently structured, are shown to be non-monophyletic. The splay-footed cricket genus Comicus, is confirmed to be genetically distinct from all Stenopelmatidae. We add two specimens to our previously published phylogenetic tree for New World Stenopelmatus Jerusalem cricket species and report the first multilocus DNA recovery for S. ater from Costa Rica. Male internal genitalia may be of systematic value in Jerusalem crickets, but we believe they should be analyzed when in their unfolded, "physiologically functional" state, where morphological characters can be seen in more detail when compared to their preserved, folded state. We describe Stenopelmatus nuevoguatemalae n. sp. from Guatemala.
Notes
Methods
We used the Orthoptera-specific target enrichment (OR-TE) probes (Shin et al. 2024) to capture 1,828 loci from genomic DNA. See Weissman et al. (2021) and Shin et al. (2024) for DNA extraction, library preparation, hybrid capture, and data processing. We inferred the phylogeny using a coalescent-based species tree analysis in the program ASTRAL 5.7.3 (Zhang et al. 2018). Specifically, we created 1,236 individual datasets (one for each gene). The gene trees were generated from each gene-specific alignment in RAxML 8 (Stamatakis 2014) using 100 rapid bootstrap pseudoreplicates and the GTR GAMMA I model. We generated a summary species tree using the default options in ASTRAL.
Files
1._phylip_files.zip
Additional details
Related works
- Is cited by
- 10.21203/rs.3.rs-3918796/v1 (DOI)
- 10.11646/zootaxa.5443.2.6 (DOI)