Detection and identification of antinuclear antibodies (ANA) in consecutive serum samples for routine autoantibody testing referred for ANA testing

Studies on autoimmune diseases in India are very less. There are scanty of research work done and data available on this topic in India. In this project work, retrospective study on data from January 2012 to December 2013 and prospective study from January 2014 to May 2014 has been done. In autoimmune illnesses, the existence, type, and strength of an autoimmune response may be gleaned from the serum auto-antibodies that are detected during diagnosis. 1441 consecutive patients (881 Female and 560 male) were evaluated prospectively. In the present study 330 (22.90%) patients were found positive for ANA and the percentage of ANA positive was found more in female 73% (241) then male 26.96%. Majority of cases showed speckled pattern 33.94% (112) followed by homogenous 26.66% (88), nucleolar 10.90% (36), cytoplasmic 2.12% (7), and 9.69% (32) showed mixed pattern.