Reproductive aging weakens offspring survival and constrains the telomerase response to herpesvirus in Pacific oysters

These datasets and codes report the impacts of aging on oyster parents and their offspring. We compared proteomic profiles (“parent_proteomic_rawdata.tsv”) of reproductive oysters (12 males and 12 females) of three age classes (young, middle age and old) (“parent_proteomic_design.txt”). We crossbred these adult oysters as described in the paper (DOI: 10.1126/sciadv.adq2311) to examine the impacts of parental aging on their offspring phenotypes. We explored the effects of reproductive aging on offspring growth (“offspring_growth_dataset.csv”) and survival rate at larval life stage from 0 to 40 days old(“larval_survival.csv”). We also measured spat resistance to OsHV1 in laboratory (“spat_mortality_OsHV1.csv”) and in the field (“spat_mortality_OsHV1_field.csv”), and offspring survival once they attained adulthood (“adult_survival.csv”). In parallel, we assayed intra- and inter-familial telomere length dynamics (“Telomere_dataset.csv”) and telomerase (“Telomerase_dataset.csv”) as described in the manuscript.

We also uploaded the R codes used to analyse proteomic data on parents (“ADupoue - Analyses code-Parent proteomic.R”) and the impacts of parental aging on their offspring traits (“ADupoue - Analyses code-Offspring.R”).

Finally, we provided the output of Markov Chain Monte Carlo models used to estimate narrow sense heritability in offspring traits (“growth_larvae.rds”, “growth_spat.rds”, “survival_larvae.rds”, “survival_OsHV1_lab.rds”, “survival_adult.rds”, “Telomere_preInf.rds”, “Telomere_postInf.rds”, “Telomerase_preInf.rds”, “Telomerase_postInf.rds”).