Biochemical and histochemical chenges in the activity of succinate dehydrogenase and ATP-ase in mitochondria of kidneys rats by acute intoxication

Dikal M. V., Ferenchuk Ye. O., Bilous Т. М., Grytsiuk M. I., Kopchuk T. G. Biochemical and histochemical chenges in the activity of succinate dehydrogenase and ATP-ase in mitochondria of kidneys rats by acute intoxication. Journal of Education, Health and Sport. 2018;8(2):263-271. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.1185391

http://ojs.ukw.edu.pl/index.php/johs/article/view/5314

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© The Authors 2018;

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This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial

use, distribution and reproduction in any medium, provided the work is properly cited.

The authors declare that there is no conflict of interests regarding the publication of this paper.

Received: 01.02.2018. Revised: 05.02.2018. Accepted: 26.02.2018.

"Pathological Physiology of Kidneys & Water Salt Homeostasis"

February, 15-16, Odessa

Materials of conference

UDC 577.152.1.008.5:616.61-008.64-099]-092.9

BIOCHEMICAL AND HISTOCHEMICAL CHANGES IN THE ACTIVITY OF SUCCINATE DEHYDROGENASE AND ATP-ASE IN MITOCHONDRIA OF KIDNEYS RATS BY ACUTE INTOXICATION

M. V. Dikal, Ye. O. Ferenchuk, Т. М. Bilous, M. I. Grytsiuk, T. G. Kopchuk

Higher State Educational Establishment of Ukraine

«Bukovinian State Medical University», Chernivtsi, Ukraine

dikalmariana@gmail.com 

Abstract

Оbject of the study was to evaluate activities of succinate dehydrogenase and ATP-ase in mitochondria of kidney under conditions of toxic influence of 2,4-dinitrophenol. The experiment was conducted on 60 male albino rats. Toxic damage was caused by 0.1% solution of 2,4-dinitrophenol intraperitoneally in the dose of 3 mg / kg. The histochemical, biochemical and statistical methods of research were used. Mitochondria isolation was performed using the method of differential centrifugation. Determination of mitochondrial succinate dehydrogenase activity consists in the restoration of potassium ferricyanide (K3[Fe (CN)6]), whose solution has a yellow color, to the colorless ferricyanide of potassium (K4[F (CN)6]) succinate by the action of succinate dehydrogenase. The activity of ATP-ase was determined by the accumulation of inorganic phosphate.

The level of activity of succinate dehidrogenase was 39% lower than that of the control values. Whereas the level of activity of ATP-ase decreased by 48% in accordance with the control. Similar histochemical changes of the enzyme of the II complex of the mitochondrial respiratory chain were revealed.

The findings revealed that 2,4-dinitrophenol produced significant biochemical and histochemical changes in the kidney of the rats. This led to a decrease enzyme activities and as a result to a discrepancy between the energy needs of the cell and energy production in the system of mitochondrial oxidative phosphorylation.

Key words: mitochondria, kidney, 2,4-dinitrophenol, succinate dehydrogenase, ATP-ase activity.