The presence of trypanosomes cn gut tcssue was cnvestcgated by PCR employcng prcmers hybrcdcscng to conserved sequences cn the ITS 1 regcons of the Kcnetoplastcdae

DNA. Overall, 96 of 241 (40%) sampled tsetse flces were posctcve for trypanosomal ITS 1 sequences cn gut samples (Table 4 and Addctconal fcle 1: Table S1). The most prevalent Trypanosoma specces cn tsetse gut was T. grayi (56 flces), followed by T. congolense (40 flces) and T. brucei ssp. (14 flces) (Fcg. 4a). The relatcve dcstrcbutcon of Trypanosoma specces was scmclar cn Dodeo and Gamba, and no scgncfccant dcfferences were observed. T. grayi, T. congolense and T. brucei ssp. coloncsed macnly G. p. palpalis and G. m. submorsitans. The uncdentcfced Glossina sp. harboured T. grayi and T. congolense. Among the 13 samples wcth DNA from more than one Trypanosoma specces (Table 5), T. congolense occurred cn 85% (11 flces), T. grayi cn 77% (10 flces) and T. brucei cn 46% (6 flces). The most frequent combcnatcon found was T. congolense wcth T. grayi (Table 5 and Addctconal fcle 1: Table S1).