IN VITRO-CYTOTOXICITY STUDIES OF METHANOLIC LEAF EXTRACT OF MEMOCYLON UMBELLATUM BURM.F. AGAINST BREAST CANCER.

The Memocylon umbellatum is a shrub widely found in the western Ghats of Karnataka ,India. The leaf extracts of the plant is being used for various medicinal purposes such as used to cure diseases Ghonerria and also used for the treatment for eye irritations. Our study was aimed to analyze the phytochemical constituents present in the methanolic leaf extract of Memocylon umbellatum and further to study its cyto-toxic activity on the MCF-7 (Breast cancer line). Extraction of phytochemicals was done by using soxhlet apparatus. The methanolic extract was tested for the preliminary phytochemical analysis by using standard methods. Test showed the presence of phytochemicals such as Tannins, Lignin, Flavanoids, Steroids, Phenols, and Glycosides. Further, the cytotoxic nature of methanolic extract was explored by performing MTT assay on MCF-7 cell line. The methanolic leaves extract of Memocylon umbellatum evidenced the inhibition of MCF-7 cancer cell lines. Thus, active compounds of the extract could be of potential medicinal application.