Published June 10, 2024 | Version v1
Journal article Open

A REVIEW OF THE UV-VISIBLE SPECTROSCOPY'S METHOD DEVELOPMENT AND VALIDATION

Description

The creation and verification of analytical methodologies are crucial to the production, development, and progression of pharmaceuticals. Pharmaceutical analysis significantly affects the quality assurance and control of both bulk drugs and pharmaceutical formulations. UV spectroscopy has been utilised in the pharmaceutical industry for the past three-and-a-half decades; it is an effective and significant analytical instrument. The processes that are required to determine the "identity, strength, quality, or purity" of these compounds are all part of the pharmaceutical analysis. The methodology for analysis involves the utilisation of colourless substances to measure how they absorb of light that is monochromatic in the near-ultraviolet (200–400 nm) range. In order to obtain the absorbance spectrum of a chemical in solution or solid form, UV-visible spectroscopy is utilised.  The creation and verification of a spectroscopy using ultraviolet light method for the precise quantification of a substance are detailed in this study. The procedure was refined by means of distinct stages, and exhaustive evaluation validated its accuracy and dependability. The outcomes demonstrate its prospective utility in various domains for accurate substance quantification.

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