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Published June 10, 2024 | Version v1
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HTS-Seg: A Dataset for Heterotypic Tumor Spheroids Segmentation

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Publication: 10.1016/j.bej.2024.109400 (DOI)

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Repository URL
https://github.com/8amirtahmasbi/HTS_Seg.git
Programming language
Python

References

  • @article{ahvaraki_advanced_2024, title = {Advanced co-culture {3D} breast cancer model to study cell death and nanodrug sensitivity of tumor spheroids}, issn = {1369-703X}, url = {https://www.sciencedirect.com/science/article/pii/S1369703X24001876}, doi = {10.1016/j.bej.2024.109400}, abstract = {The interaction between tumor cells and other cells in the tumor microenvironment (TME) dramatically affects the behavior and progression of the disease. Fibroblasts, as a major component of TME, play a role in cancer development and drug resistance. The current study demonstrates a microfluidic-based co-culture 3D breast cancer spheroids with simplified on-chip testing. To better mimic the breast TME, we have successfully generated a 3D stroma-rich in vitro model of breast cancer using fibroblasts and breast cancer cells. Using fluorescence imaging and histology techniques, the developed spheroids were characterized. MDA-MB-231 cells were successfully grown into 3D spheroids. Upon co-culturing the cells, a decrease in spheroid diameter was detected. Furthermore, tumor spheroids' overall viability was increased when co-cultured with fibroblasts over 5 days. The results demonstrated an increase in ECM content in co-culture spheroids (heterotypic spheroids) compared to mono-culture spheroids (homotypic spheroids), as demonstrated by the augmentation in the quantity of blue stain all over the spheroids. When exposed to liposomal doxorubicin nanodrug, a survival advantage was noticed in heterotypic spheroids compared to mono-culture spheroids (91\% vs. 82\%). The results highlighted the significance of using 3D tumor models rather than 2D systems as a realistic platform for proper evaluation of therapeutics efficacy. The co-culture tumor spheroids in proposed microwell-based microfluidic devices may be valuable to investigate TME and drug screening.}, urldate = {2024-06-19}, journal = {Biochemical Engineering Journal}, author = {Ahvaraki, Akram and Gheytanchi, Elmira and Behroodi, Ebrahim and Latifi, Hamid and Vakhshiteh, Faezeh and Bagheri, Zeinab and Madjd, Zahra}, month = jun, year = {2024}, keywords = {Co-cultured spheroid, Fibroblast, Liposomal doxorubicin, Microfluidics}, pages = {109400}, file = {ScienceDirect Snapshot:C\:\\Users\\Amir\\Zotero\\storage\\Q3BAGDNH\\S1369703X24001876.html:text/html}, }