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Published June 4, 2024 | Version 1.0

The polyadenylation landscape after in vivo long-term potentiation in the rat brain

Description

Supplementary Table 1. Summary of dentate gyri DRS data per gene. (A) Differential expression and differential adenylation data for 10 min timepoint. (B) Differential expression and differential adenylation data for 60 min timepoint. (C) GO-terms for genes with significantly elongated poly(A) tails in 10 min timepoint. (D) GO-terms for genes with significantly elongated poly(A) tails in 60 min timepoint. (E) GO-terms for upregulated genes in 10 min timepoint. (F) GO-terms for upregulated genes in 60 min timepoint. (G) GO-terms for upregulated genes with CPEB-binding motifs in 10 min timepoint. (H) GO-terms for upregulated genes with CPEB-binding motifs in 60 min timepoint.

Supplementary Table 2. Summary of dentate gyri cDNA data per gene. (A) Differential expression and differential adenylation data for 10 min timepoint. (B) Differential expression and differential adenylation data for 60 min timepoint. (C) GO-terms for genes with significantly elongated poly(A) tails in 10 min timepoint. (D) GO-terms for genes with significantly elongated poly(A) tails in 60 min timepoint. (E) GO-terms for upregulated genes in 10 min timepoint. (F) GO-terms for upregulated genes in 60 min timepoint. (G) GO-terms for upregulated genes with CPEB-binding motifs in 10 min timepoint. (H) GO-terms for upregulated genes with CPEB-binding motifs in 60 min timepoint.

Supplementary Table 3. High-confidence PASs. (A) PASs predicted for datasets obtained 10 min after LTP induction by TAPAS. (B) High confidence poly(A) clusters predicted by LAPA for datasets obtained 10 min after LTP induction. (C) PASs predicted for datasets obtained 60 min after LTP induction. (D) High confidence poly(A) clusters predicted by LAPA for datasets obtained 60 min after LTP induction.

Supplementary Table 4. Nonadenosine profiling upon LTP induction. (A) Summary of Ninetails pipeline for dentate gyrus. (B) List of genes containing semi-templated poly(A) tails with their 3UTR coordinates.

Supplementary Table 5. Summary of synaptoneurosomal DRS data per gene. Differential expression and differential adenylation data.

Supplementary Table 6. Characteristics of analyzed material. Detailed information with sequencing depth, accession numbers, software used for analyses, and additional data characteristics.

Supplementary Resource High-confidence poly(A) clusters annotation in bed format.

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Additional details

Software

Repository URL
https://github.com/LRB-IIMCB/ninetails
Programming language
R , Python
Development Status
Active