Surveying the fauna in conservation areas is an important strategy to recognize patterns of richness and endemism and to reduce gaps related to geographical distribution. This study presents the first record of cladocerans in the Parque Nacional do Itatiaia, Southeastern Brazil. Alona ossiani, Alona guttata, Alona iheringula, Alona yara, and Briospylus repens were observed in different environments. Here, we provide the taxonomic status and geographic distribution of each species observed in the Parque Nacional do Itatiaia.