A Study of Different types of Hemoglobinopathies in Pediatric Population with Anemia by High Performance Liquid Chromatography in a Tertiary Care Centre
Authors/Creators
- 1. Junior Residence, Department of Pathology, Rajshree Medical Research Institute, Bareilly, India
- 2. Professor, Department of Pathology, Rajshree Medical Research Institute, Bareilly, India
- 3. Assistant Professor, Department of Pathology, Rajshree Medical Research Institute, Bareilly, India
- 4. Professor and HOD, Department of Pathology, Rajshree Medical Research Institute, Bareilly, India
- 5. Professor and HOD, Department of Paediatric, Rajshree Medical Research Institute, Bareilly, India
Description
Background: Hemoglobinopathies are the most common inherited red cell disorders worldwide. Identification of these disorders is immensely important epidemiologically and for improved management protocols. Among these Hemoglobinopathies, Thalassemia and Sickle Cell Anemia constitute major public health problems. Aim: To study the prevalence of Hemoglobinopathies in anemic pediatric population by using HPLC. Materials and Methods: This study was done at our tertiary care center, from January 2021 to July 2022. Hematological indices were derived from hematology analyzer (Mindray BC-5150) and Hemoglobin Electrophoresis was carried out by D-10 (BIO-RAD). Results: Out of 378 cases 33 (8.73%) cases had Hemoglobinopathies. The most predominant hemoglobinopathy was of Thalassemia cases 27 (7.76%) followed by Sickle Cell disorders cases 4(1.06%) with slight male preponderance, 20 were male and 13 were female. Conclusion: An extensive screening of the population is important to assess the prevalence of hemoglobinopathies, which will help in identification of carriers and take adequate therapeutic and preventive measures. Cation Exchange HPLC is emerging as one of the best methods for screening and detection of various Hemoglobinopathies with rapid, reproducible and precise results.
Abstract (English)
Background: Hemoglobinopathies are the most common inherited red cell disorders worldwide. Identification of these disorders is immensely important epidemiologically and for improved management protocols. Among these Hemoglobinopathies, Thalassemia and Sickle Cell Anemia constitute major public health problems. Aim: To study the prevalence of Hemoglobinopathies in anemic pediatric population by using HPLC. Materials and Methods: This study was done at our tertiary care center, from January 2021 to July 2022. Hematological indices were derived from hematology analyzer (Mindray BC-5150) and Hemoglobin Electrophoresis was carried out by D-10 (BIO-RAD). Results: Out of 378 cases 33 (8.73%) cases had Hemoglobinopathies. The most predominant hemoglobinopathy was of Thalassemia cases 27 (7.76%) followed by Sickle Cell disorders cases 4(1.06%) with slight male preponderance, 20 were male and 13 were female. Conclusion: An extensive screening of the population is important to assess the prevalence of hemoglobinopathies, which will help in identification of carriers and take adequate therapeutic and preventive measures. Cation Exchange HPLC is emerging as one of the best methods for screening and detection of various Hemoglobinopathies with rapid, reproducible and precise results.
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Additional details
Dates
- Accepted
-
2023-01-05
Software
References
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