Published May 2, 2024
| Version v4
Journal article
Open
Chromatin context-dependent effects of epigenetic drugs on CRISPR-Cas9 editing
Authors/Creators
-
1.
The Netherlands Cancer Institute
-
2.
Oncode Institute
-
3.
University of Milan
-
4.
Eindhoven University of Technology
Description
The efficiency and outcome of CRISPR/Cas9 editing depends on the chromatin state at the cut site. It has been shown that changing the chromatin state can influence both the efficiency and repair outcome, and epigenetic drugs have been used to improve Cas9 editing. However, because the target proteins of these drugs are not homogeneously distributed across the genome, the efficacy of these drugs may be expected to vary from locus to locus. Here, we systematically analyzed this chromatin context-dependency for 160 epigenetic drugs.
In this repository, laboratory notebook prints and supplementary data such as gel images are deposited. Processed data and data analysis pipelines are deposited in the affiliated GitHub release on Zenodo.
Files
labguru_3.zip
Files
(305.2 MB)
| Name | Size | Download all |
|---|---|---|
|
md5:b9df4c024dfe53a98df3a8b460fad95f
|
305.2 MB | Preview Download |
Additional details
Related works
- Is supplemented by
- Software: 10.5281/zenodo.11103982 (DOI)
- Dataset: PRJNA960840 (Other)
- Dataset: GSE248980 (Other)