Fragmentation and aggregation of cyanobacterial colonies under a cone and plate shear

Primary data associated with the publication:

Yuri Z. Sinzato, Robert Uittenbogaard, Petra M. Visser, Jef Huisman, Maziyar Jalaal, 2025, Fragmentation and aggregation of cyanobacterial colonies, eLife 14:RP103503,

https://doi.org/10.7554/eLife.103503.3

Brief description of experimental setup: 
Colonies of the cyanobacteria Microcystis in liquid medium BG-11 are place and a cone-and-plate shear at various values of energy dissipation rate and total biovolume fraction. Brightfield microscopy is perfomed simulanoeusly to the shear flow thorugh the transparente lower plate.

Files:
rawimages.zip: Raw images (.tiff) for the dataset Fragmentation-eps-5.8m2/s3-phi100ppm. Pixel resolution is 1.63 um/px.
features.zip : Processed data from raw images for all data sets are present. Title inform initial distribution ("Bre" = large colonies; "Agg" = single cells), nominal shear rate and total biovolume fraction. Csv files contain background image, detected features using a routine in scikit-image scikit-image and initial parameters. Script for analizing data can be found at https://github.com/FluidLab/CyanoB_Agg_Frag.git

Contact: Yuri Sinzato - y.z.sinzato@uva.nl / Mazi Jalaal - m.jalaal@uva.nl