STANDARDISATION OF BIOACTIVE MATERIALS FROM CHROMULINA FREIBERGENSISAGAINST HUMAN PATHOGENS USING GC-MS ANALYSIS

In the preceding ten years, there has been a surge in the distribution of secondary metabolites and pharmacologically dynamic compounds from marine microalgae. Chromulinafreibergensis provided the antibacterial metabolites lessons for the current research .The current secondary metabolites study was chosen to focus on the green algal Chromulinafreibergensis. To see whether the properties of pH, temperature, and salinity on the development of microalgae aided in the growth of microalgae, they were tested. Antibacterial activity against Vibrio cholerae, Klebsiella pneumonia, Escherichia coli, Pseudomonas aeruginosa, Salmonella sp., Proteus sp., Streptococcus pyrogens, Staphylococcus aureus, Bacillus megaterium, and Bacillus subtilis was tested for in different solvent extracts of Chromulinafreibergensis.On the ninth day of incubation, when the medium was adjusted to a pH of 9.0 in 30 ppt of salinity at 250C, the uppermost cell growth was noticed. Of all the investigated solvents, the butanol + isopropanol (1:1) crude extract of Chromulinafreibergensis tested the highest zone of inhibition (13.4 mm) against Salmonella. . The presence of rare chemical substances like 3, 3, 5-Trimethylheptane (M.W. 142.2) and n-Hexadecane (M.W. 226.2) in the crude extract of Chromulinafreibergensis was discovered using GC-MS analysis.These results demonstrated the strong antibacterial activity of the Chromulinafreibergensis Butanol + Isopropanol (1:1) extract, demonstrating its enormous potential as a solvent for the extraction of bioactive chemicals from natural sources for biomedical and pharmaceutical applications.