Code to reproduce the analyses of "Multiple stressors alter greenhouse gas concentrations in streams through local and distal processes"

Streams are significant contributors of greenhouse gases (GHG) to the atmosphere, and the increasing number of stressors degrading freshwaters may exacerbate this process, posing a threat to climatic stability. However, it is unclear whether the influence of multiple stressors on GHG concentrations in streams results from increases of in-situ metabolism (i.e., local processes) or from changes in upstream and terrestrial GHG production (i.e., distal processes). Here, we hypothesize that the mechanisms controlling multiple stressor effects vary between carbon dioxide (CO₂) and methane (CH₄), with the latter being more influenced by changes in local stream metabolism, and the former mainly responding to distal processes. To test this hypothesis, we measured stream metabolism and the concentrations of CO₂ (pCO₂) and CH₄ (pCH₄) in 50 stream sites that encompass gradients of nutrient enrichment, oxygen depletion, thermal stress, riparian degradation and discharge. Our results indicate that these stressors had additive effects on stream metabolism and GHG concentrations, with stressor interactions explaining limited variance. Nutrient enrichment was associated with higher stream heterotrophy and pCO₂, whereas pCH₄ increased with oxygen depletion and water temperature. Discharge was positively linked to primary production, respiration and heterotrophy but correlated negatively with pCO_2. Our models indicate that CO₂-equivalent concentrations can more than double in streams that experience high nutrient enrichment and oxygen depletion, as compared to those with oligotrophic and oxic conditions. Structural equation models revealed that the effects of nutrient enrichment and discharge on pCO₂ were related to distal processes rather than local metabolism. In contrast, pCH₄ responses to nutrient enrichment, discharge and temperature were related to both local metabolism and distal processes. Collectively, our study illustrates potential climatic feedbacks resulting from freshwater degradation and provides insight into the processes mediating stressor impacts on the production of GHG in streams.