PREPARATION AND CHARACTERIZATION OF NATURAL ANTIACNE CREAM CONTAINING PLANT EXTRACT

Many diseases are produced due to accumulation of free radicals in the body. The aim of the present study were to evaluate in vitro antioxidant activities. The extract was prepared by using pure ethanol solvent for Cardiospermum halicacabum aerial part. The in vitro antioxidant activity of Cardiospermum halicacabum aerial part extract was evaluated by using different methods like nitric oxide scavenging activity, free radical scavenging by DPPH method and scavenging of hydroxyl radical (Deoxyribose Method). Vitamin-E was used as standard. The tested plant extract in different concentration showed promising antioxidant and free radical scavenging activity, Concentration 200 µg showed best antioxidant activity compared to other concentration in nitric oxide activity. In DPPH method Concentration 400µg showed maximum antioxidant activity compared to other concentration. From the obtained results we could suggest that this plant extract can be used for antioxidant activity and suggest to go for In-vivo studies.

Key words: Ethanolic plant extract, In vitro antioxidant activity, Nitric oxide, DPPH, Deoxyribose method.