HindaNajem/CellPress_STARProtocols: Quantification of immune cell distribution from the vasculature to the glioma microenvironment on sequential immunofluorescence multiplex

Except for microglia, immune surveillance, and infiltration into the glioma tumor microenvironment (TME) arise from the periphery. Although myeloid-derived immune cells can be dispersed throughout the TME, anti-tumor effector cells are confined to the perivascular space. Therapeutics that induce the dispersal of effector cells throughout the TME would be advantageous. This bioinformatic analysis of multiplex images quantifies immune distribution in the glioma TME as function of vessel proximity and will be used in the analysis of prognosis and immunotherapy clinical trials.