Published March 20, 2024 | Version v1
Conference paper Open

Electrochemical Detection of MMP-2 Using Graphene-Based Aptasensor

  • 1. BioSense Institute
  • 2. ROR icon TU Wien
  • 3. Vienna University of Technology

Description

A graphene-based electrochemical biosensor was developed for the detection of matrix metalloproteinase 2 (MMP-2) endopeptidase, whose expression can be significantly related to the occurrence, metastasis, and prognosis of cancer. A specific anti-MMP-2 aptamer was successfully immobilized on the surface of electrochemically reduced graphene oxide via a pyrene-based linker, enabling the specific capture of MMP-2. The sensor was able to detect 1 ng mL−1, with an overall detection time of less than 20 min. Moreover, the aptamer-based biosensor showed good specificity toward different unspecific proteins.

Files

proceedings-97-00057.pdf

Files (474.4 kB)

Name Size Download all
md5:c0affa73fad7971315232bc426451baa
474.4 kB Preview Download

Additional details

Dates

Accepted
2024-03-20