OPTIMIZATION AND FORMULATION OF ERIBULIN BASED SILVER NANOPARTICLES LOADED MICROEMULSION FOR TARGETED ORAL DELIVERY SYSTEM: A COST-EFFECTIVE AND ECO-FRIENDLY APPROACH WITH DUAL ANTICANCER AND ANTIBACTERIAL PROPERTIES

Abstract

The most frequent sickness to affect women is breast cancer. A targeted drug delivery strategy for an anticancer agent is required to treat breast cancer (BC) cells to the appropriate therapeutic potential without endangering healthy cells. The main objective of this work is to optimise and synthesise silver nanoparticles (AgNPs) for oral usage in a cost-effective and ecologically friendly way. Furthermore, using animal models and cancer cell lines, AgNPs laden ME will be examined for antibacterial and anticancer properties. Eribulin is a synthetic medication having potent anticancer effects against breast cancer. A neural network (NN) optimisation technique was used to synthesise AgNPs and determine the relationship between the formulation elements and response variables (AgNPs size). For the improved AgNPs in the AgNPs loaded ME formulation, evaluations were conducted regarding particle size and shape, morphological characterisation, particle charge, and in vitro drug release investigations. The 3-factor, D-optimal mixture model and the pseudo-ternary phase diagram were used for the synthesis and evaluation of AgNPs loaded ME. The microemulsion containing silver nanoparticles was evaluated for its physicochemical properties, particle size, shape, surface morphology, zeta potential and in vitro release assays. AgNPs loaded ME exhibit active drug release, which increases the drug's therapeutic concentration and bioavailability in cancer cells compared to healthy cells. In addition to having great anticancer potential against MCF-7 cancer cells, the synthetic AgNPs in the AgNPs-loaded microemulsion may also be able to stop the growth of bacteria. The in-vivo study revealed that, in comparison to the tumor control, swallowing AgNPs-loaded ME formulations dramatically decreased the tumor mass and Ehrlich ascites solid tumor development rate in the mice. The results indicate that AgNPs in AgNPs loaded ME appear promising as an anticancer therapy in terms of stability, cost, and simplicity of fabrication.