Fig. 4. Effects of direct transfer from FW to SW on protein abundance (A) and activity (B) of Na+/K+-ATPase (NKA) in kidneys of the green spotted pufferfish. The asterisks indicate significant differences (P <0.05) using Dunnett's multiple-comparison test following a one-way ANOVA. Values are means ± SEM (n = 5). NKA protein decreases gradually within 6 h post-transfer and significantly decreases after 12 h post-transfer. NKA activity decreases significantly within 3 h post-transfer returns to the initial level at 6 h, and decreases significantly after 12 h post-transfer.