BDL surgery protocol

Protocol for bile duct ligation surgery (BDL) in adult and young (pups) rats performed in the MRS4Brain research group

By:

Dr.med.vet. Stefanita-Octavian Mitrea (stefan.mitrea@epfl.ch, CIBM Center for Biomedical Imaging, Lausanne, Switzerland; Animal Imaging and Technology, Ecole Polytechnique Federale de Lausanne (EPFL), Lausanne, Switzerland)

Dario Sessa (dario.sessa@epfl.ch, Swiss Pediatric Liver Center, Department of Pediatrics, Gynecology and Obstetrics, University of Geneva, Geneva, Switzerland.)

after a protocol of Dr.Graziano Oldani (Division of Abdominal and Transplantation Surgery, Department of Surgery, Faculty of Medicine, Geneva University Hospitals, Geneva, Switzerland; Faculty of Medicine, Hepato-pancreato-biliary Centre, Geneva University Hospitals, Geneva, Switzerland)

Please cite the following manuscripts if you ar using the protocol described herein:

For adult rats:

Longitudinal neurometabolic changes in the hippocampus of a rat model of chronic hepatic encephalopathy - PubMed (nih.gov)

Probiotics improve the neurometabolic profile of rats with chronic cholestatic liver disease - PubMed (nih.gov)

Probiotics combined with rifaximin influence the neurometabolic changes in a rat model of type C HE - PubMed (nih.gov)

PET CMRglc mapping and 1H-MRS show altered glucose uptake and neurometabolic profiles in BDL rats - PubMed (nih.gov)

Central nervous system and systemic oxidative stress interplay with inflammation in a bile duct ligation rat model of type C hepatic encephalopathy - PubMed (nih.gov)

For rat pups:

Longitudinal osmotic and neurometabolic changes in young rats with chronic cholestatic liver disease - PubMed (nih.gov)

Neurometabolic changes in a rat pup model of type C hepatic encephalopathy depend on age at liver disease onset - PubMed (nih.gov)