Images of mouse VTA and SNc sections labeled for neurotransmitter phenotype markers via RNAscope

Count graphics are named according to transporter(s) (e.g. "vmat2" or "vmat2 and vgat"). Note that these are not markings of cell types, rather just transporter expression. For example, neurons labeled "vmat2" belong to all cell types with VMAT2: vmat2 only, vmat2 + vgat only, vmat2 + vglut2 only, and triple. 

Sometimes multiple count graphics for a single transporter show up (e.g. "vmat2 part2" or "vmat2 addition". The additional graphics are for neurons that were missed in the initial count due to accidental exiting of the Count tool.

Sometimes an extra event counter was created or placed in the wrong location. In these cases the graphic will have a note saying how many miscounts there are (e.g. "VMAT2 -1" for one miscount). There will be a correction graphic (a black rectangle) after covering the miscount (e.g. "VMAT2 correction").

T1: VMAT2
T2: VGAT
T3: VGLUT2
T4: DAPI

Animals alpha, epsilon, and mu are male; beta, sigma, and delta are female. All were 8-9 weeks old at time of sacrifice.

Fiber regions:
ml: medial lemiscus
fr: fasciculus retroflexus
mt: medial terminal nucleus of the accessory optic tract
pm: principal mammilary tract 
vtgx: ventral tegmental decussation

Cellular regions:
CLi: Caudal linear nucleus 
DR: Dorsal raphe
IF: Interfascicular nucleus 
IPN: Interpeduncular nucleus
PBP: Parabrachial pigmented nucleus 
PIF: Parainterfascicular nucleus
PN: Paranigral nucleus 
RLi: Rostral linear nucleus
RN: red nucleus 
SNCD: SNc, dorsal
SNCL: SNc, lateral 
SNCM: SNc, medial
SNCV: SNc, ventral 
VTAR: VTA, rostral