Widefield and Confocal brain images derived from experimental Middle Cerebral Artery occlusion (MCAo) in mice.

This deposit contains a series of widefield and confocal brain images derived from research on cerebral ischemia carried out by the Laboratory of Neurovascular Interactions (https://elalilab.com/) at the Université Laval, Québec (Canada) directed by Dr. Ayman ElAli (ayman.el-ali@crchudequebec.ulaval.ca). The experiments, data processing, and organization were performed by Daniel Manrique-Castano, former postdoctoral fellow and current research data curation officer at the Digital Research Alliance of Canada (https://daniel-manrique.github.io/manrique; dmanriquecastano@gmail.com).

Given the complexity of the whole image bundle, images from the same experiment were compressed into zip files. Complementary data is available at the Open Science Framework (OSF) repository (10.17605/OSF.IO/74MQN), and reproducible workflows for image processing and analysis are available on GitHub (10.5281/zenodo.13948420)

Naming conventions for datasets (taking as an example Widefield_5x_Ipsilateral_KO_CD31-ColIV-Iba1.zip)

Widefield: Imaging type performed (available strings = Confocal, Widefield). 

5x: Objective magnification (Available strings = 5x, 10x, 20x, 40x). 

Ipsilateral: Iaged region (Available strings = ROIs [Regions of interest], Whole [whole brain], Ipsilateral [ischemic hemisphere]). 

KO: imaging performed in PDGFRB_KLF4-KO mice (if absent, imaging performed in PDGFRB_TdTomato mice) 

CD31-ColIV-Iba1: Imaged markers (available strings = multiple, depending on the experiment). 

Important: Each .zip file contains a README file specific to each dataset, describing the experimental conditions, naming conventions of individual images and links to additional files used for image processing.