Studies on Bioemulsifier producing Bacteria

Abstract:

There is an increasing enthusiasm for the creation and utilization of natural emulsifiers that offer both biodegradability and non-toxic properties, surpassing the performance of current emulsifying agents employed in the food industry. A wide array of microorganisms can serve as viable sources of raw materials, providing a diverse range of applications. This study focuses on the isolation and identification of bacterial strains capable of producing bioemulsifiers from hydrocarbon-contaminated sites. The objective is to assess their bioemulsifying properties. Screening was conducted using the drop-collapse method and minimal agar supplemented with a diesel layer as a carbon source. Positive strains, demonstrating growth in a liquid medium, were identified. Out of the 10 bacterial isolates tested, 2 exhibited positive results using both the drop-collapse and hydrocarbon layer agar methods. Morphological and biochemical characterization was employed for identification, revealing the isolates to be Bacillus sp. and Escherichia coli.