Published February 29, 2016
| Version v1
Taxonomic treatment
Open
Karenia brevis subsp. homogenate
Authors/Creators
- 1. Department of Chemistry and Biochemistry, Florida International University, Miami, FL 33199, USA
Description
4.3. Preparation of K. brevis homogenate
K. brevis culture (200 ml) was concentrated by centrifugation (5 min at 450× g) and the supernatant discarded. The cells were resuspended in phosphate buffer (800 Ll, 50 mM pH 7.0) and vortexed for 1 min. This suspension was centrifuged (14,000× g for 10 min) and the pellet was discarded. Homogenates were analyzed for protein concentration and glucose-6-phosphate dehydrogenase activity as described below. Typical protein concentrations ranged from (0.25 to 0.5 mg /ml).
Notes
Published as part of Sun, Pengfei, Leeson, Cristian, Zhi, Xiaoduo, Leng, Fenfei, Pierce, Richard H., Henry, Michael S. & Rein, Kathleen S., 2016, Characterization of an epoxide hydrolase from the Florida red tide dinoflagellate, Karenia brevis, pp. 11-21 in Phytochemistry 122 on page 18, DOI: 10.1016/j.phytochem.2015.11.002, http://zenodo.org/record/10485359
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Linked records
Additional details
Identifiers
Biodiversity
- Kingdom
- Animalia
- Phylum
- Arthropoda
- Order
- Hemiptera
- Family
- Cicadidae
- Genus
- Karenia
- Species
- homogenate
- Taxon rank
- subSpecies