Graph Fourier transform for spatially resolved omics representation and analyses

The file is a CODEX image of a human tonsil. They contain:

1. Whole image: entire tissue image of human tonsil.
2. Raw image data: six field of view (FOV) are deposited in this figure
3. Processed data: the pixel-level data of six FOVs are deposited in the Seurat object and H5AD file.

The following anti-bodies were used for CODEX profiling:

BCL-2 (124, Novus Biologicals), CCR6 (polyclonal, Novus Biologicals), CD11b (EPR1344, Abcam), CD11c (EP1347Y, Abcam), CD15 (MMA, BD Biosciences), CD16 (D1N9L, Cell Signaling Technology), CD162 (HECA-452, Novus Biologicals), CD163 (EDHu-1, Novus Biologicals), CD2 (RPA-2.10, Biolegend), CD20 (rIGEL/773, Novus Biologicals), CD206 (polyclonal, R&D Systems), CD25 (4C9, Cell Marque), CD30 (BerH2, Cell Marque), CD31 (C31.3+C31.7+C31.10, Novus Biologicals), CD4 (EPR6855, Abcam), CD44 (IM-7, Biolegend), CD45 (B11+PD7/26, Novus Biologicals), CD45RA (HI100, Biolegend),  CD45RO (UCH-L1, Biolegend), CD5 (UCHT2, Biolegend), CD56 (MRQ-42, Cell Marque), CD57 (HCD57, Biolegend), CD68 (KP-1, Biolegend), CD69 (polyclonal, R&D Systems), CD7 (MRQ-56, Cell Marque), CD8 (C8/144B, Novus Biologicals), collagen IV (polyclonal, Abcam), cytokeratin (C11, Biolegend), EGFR (D38B1, Cell Signaling Technology), FoxP3 (236A/E7, Abcam), granzyme B (EPR20129-217, Abcam), HLA-DR (EPR3692, Abcam), IDO-1 (D5J4E, Cell Signaling Technology), LAG-3 (D2G4O, Cell Signaling Technology), mast cell tryptase (AA1, Abcam), MMP-9 (L51/82, Biolegend), MUC-1 (955, Novus Biologicals), PD-1 (D4W2J, Cell Signaling Technology), PD-L1 (E1L3N, Cell Signaling Technology), podoplanin (D2-40, Biolegend), T-bet (D6N8B, Cell Signaling Technology), TCR β (G11, Santa Cruz Biotechnology), TCR-γ/δ (H-41, Santa Cruz Biotechnology), Tim-3 (polyclonal, Novus Biologicals), Vimentin (RV202, BD Biosciences), VISTA (D1L2G, Cell Signaling Technology), α-SMA (polyclonal, Abcam), and β-catenin (14, BD Biosciences).