We report the first documented record of Acomys dimidiatus (Cretzschmar, 1826) from Lebanon. An opportunistic trapping was carried on in Hima Ibl Al Saqi, southern Lebanon for five consecutive nights during the spring and fall of 2022. Four spiny mice were trapped, and their morphometric measurements were recorded. These measurements resemble those documented for this species in other parts of the Middle East. Our new record extends the known distribution of Acomys dimidiatus approximately 45 km away from its most northern known geographic range.