Exploring Cellular Dynamics: A Comprehensive Analysis of SH-SY5Y Cell Growth in Variable Polycarbonate Chambers and Comparative Insights with Standard Petri Dishes

The file nomenclature employed herein serves to represent the primary experimental conditions under consideration. It comprises a hierarchical structure, encompassing various parameters as follows: cellsName_passageNumber_cellSuspension_measurementDayNumber_chamberDiameter_regionNumber_hOfFluids_number.

The cellsNames parameter encapsulates the complete nomenclature of the cells utilized in the experiment.

The passageNumber parameter represents the passage number and is encoded as 'pNumber.'

The cellSuspension parameter is denoted by distinct codes:

• 'c1' designates a cell suspension with a concentration of 5x10^5 cells/ml.
• 'c3' signifies a cell suspension with a concentration of 1x10^5 cells/ml.
• 'c4' denotes a cell suspension with a concentration of 5x10^4 cells/ml.

The measurementDayNumber parameter is encoded as 'afterXXXdays,' signifying the number of culture days.

The 'd' followed by a number (e.g., d6, d8, d10, d12, d15) indicates the diameter of the chamber in millimeters. In a given series of files (e.g., 1day_d6_1, 1day_d6_2, 1day_d6_3, 1day_d6_4, 1day_d6_5), the trailing numeral (_1, _2, _3, _4, _5) corresponds to a photograph captured in a distinct region of the sample.

The hOfFluids parameter is employed to signify the height ('h') of the fluid above the cells in the chamber, expressed in millimeters.