Published April 4, 2017 | Version v1
Journal article Open

XTEND: Extending the depth of field in cryo soft X-ray tomography

Authors/Creators

  • 1. Centro Nacional de Biotecnología (CNB-CSIC), Cantoblanco, Madrid, 28049, Spain
  • 2. ALBA Synchrotron Light Source, Cerdanyola del Vallès, Barcelona, 08290, Spain
  • 3. Centro Nacional de Microbiología, ISCIII, Majadahonda, Madrid, 28220, Spain
  • 4. Department of Computer Science, The Graduate Center, City University of New York, NY 10016, USA
  • 5. Escuela Politécnica Superior, Univ. Autónoma de Madrid, Cantoblanco, Madrid, 28049, Spain

Description

We have developed a new data collection method and processing framework in full field cryo soft X-ray tomography to computationally extend the depth of field (DOF) of a Fresnel zone plate lens. Structural features of 3D-reconstructed eukaryotic cells that are affected by DOF artifacts in standard reconstruction are now recovered. This approach, based on focal series projections, is easily applicable with closed expressions to select specific data acquisition parameters.

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Additional details

Funding

European Commission
BIOSTRUCT-X - Transnational access and enhancement of integrated Biological Structure determination at synchrotron X-ray radiation facilities 283570
European Commission
iNEXT - Infrastructure for NMR, EM and X-rays for translational research 653706