Published November 21, 2023 | Version v1
Journal Open

Projective light-sheet microscopy with flexible parameter selection

Creators

  • 1. ROR icon Southwestern Medical Center
  • 2. ROR icon University of Chicago

Description

Projection imaging accelerates volumetric interrogation in fluorescence microscopy, but for multi-cellular samples, the resulting images may lack contrast, as many structures and haze are summed up. Here, we demonstrate rapid projective light-sheet imaging with parameter selection (props) of imaging depth, position and viewing angle. This allows us to selectively image different sub-volumes of a sample, rapidly switch between them and exclude background fluorescence. We demonstrate the power of props by functional imaging within selected, distinct regions of the zebrafish brain, firing of calcium inside muscle cells of moving Drosophila larvae, super-resolution imaging of selected cell layers, and by optically unwrapping the curved surface of a Drosophila embryo. We anticipate that props will accelerate volumetric interrogation, ranging from sub-cellular to mesoscopic scales.

Files

Files (36.2 GB)

Name Size Download all
md5:bb200f9f6ebd06723534d881c102bf94
25.5 MB Download
md5:9ff043f5cc17b56c2fc6e7fde4425fbc
229.3 MB Download
md5:0ba8646be96b56333b966465a4a707b8
109.6 MB Download
md5:3e27f00510a6d9377869b630e0c712cb
78.7 MB Download
md5:eb82b3a2fefb68a417b2e115f5983bf6
949.0 MB Download
md5:6885631b90c1f2347dd772d4641a809c
22.2 MB Download
md5:e297fe9f5783f2532b8d874e420b891d
5.0 GB Download
md5:83bdb8b93d2b5ca900b7cdd2512421c4
1.1 GB Download
md5:2b37ef2527e192240d99b178b3ea651a
3.4 GB Download
md5:150437523aff7ff4d7dedf378ed1975b
20.1 GB Download
md5:6b527a2a93bec2e4677ef02479b9d8a8
4.0 GB Download
md5:a32a074ba249418af59176ce52578f13
780.3 MB Download
md5:208fe3e24b642f3abc2d93d264e27ba2
261.7 MB Download

Additional details

Funding

National Institutes of Health
Transformative microscopes to image across spatiotemporal scales R35GM133522