Copper Nanoparticles Phytofabrication by Utilizing Brassica oleracea var. gemmifera and Exploring their Potential as Antioxidant and Antibacterial Agents

The study aimed to synthesize copper nanoparticles (CuNPs) using Brassica oleracea var. gemmifera extract (BOE) and investigate their  antioxidant and antibacterial properties. The BOE- mediated CuNPs (BOE-CuNPs) were successfully  synthesized utilizing reducing and stabilizing properties of B. oleracea var. gemmifera extract. The nanotechnology features showed that BOE-CuNPs were nano in size (106.59 ± 4.77 nm), slightly agglomerated, stable (-12.41 ± 1.88 mV), and made up of mainly copper. The green synthesized BOE-CuNPs have potential antioxidant features and have EC50 values (concentration essential to inhibit 50% of free radicals) of 334.09 ± 17.70 μg/mL and 361.43 ± 12.91 μg/mL in DPPH and ABTS free radical scavenging activity, correspondingly, and could be useful in treating oxidative-stress-mediated conditions. Also, as-synthesized BOE-CuNPs have potential antibacterial activity and showed the highest antibacterial activity against Escherichia coli – MTCC 41 and Listeria monocytogenes – MTCC 657, and could be useful as an antibacterial representative in the  biomedical field. However, to utilize BOE-CuNPs, an in- detailed functional and toxicological profile of BOE- CuNPs needs to be performed.

Keywords:- Copper Nanoparticles, Brassica oleracea var. gemmifera, Green Synthesis, Antioxidant Activity, Antibacterial Activity.