INVESTIGATIONS OF HERBAL EXTRACTS USING ANIMAL MODELS FOR ANTIDEPRESSANTS AND ANTI-ANXIETY ACTIVITIES

The aim of this dissertation was to investigate the potential antidepressant effects of the methanolic extract of Sagittaria Trifolia and Litchi Chinensis in a mouse model. The study focused on assessing behavioral changes and antioxidant properties of the extract in order to provide insights into its therapeutic potential.The primary objectives included evaluating the antidepressant-like effects through behavioral assessments using the Forced Swim Test, Tail Suspension Test, and Open Field Test. Additionally, the study aimed to determine the antioxidant capacity of the extract and investigate its potential mechanism of action.Mice were treated with the methanolic extract of Sagittaria Trifolia and Litchi Chinensis for 14 days, followed by behavioral assessments on days 1, 7, and 14. Four groups were formed, including a control group, a standard group treated with Imipramine, and two test groups treated with different doses of the extract. Biochemical parameters, such as serotonin, norepinephrine, catalase activity, lipid peroxidation, superoxide dismutase (SOD) activity, and reduced glutathione (GSH), were measured to assess the extract's impact on the central nervous system and antioxidant capacity.The results of this study suggest that the methanolic extract of Sagittaria Trifolia and Litchi Chinensis exhibits potential antidepressant-like effects in mice, as indicated by reduced immobility duration in behavioral tests, particularly at the higher dose. Moreover, the extract demonstrated promising antioxidant properties, with increased catalase activity and reduced lipid peroxidation. While these findings are encouraging, further research is needed to elucidate the precise mechanisms of action and to assess the safety and efficacy of Sagittaria Trifolia and Litchi Chinensis extract for potential clinical use. This study contributes to the understanding of natural product-based alternatives for depression treatment, emphasizing the need for continued investigation into the therapeutic potential of this plant extract.

Keywords:Sagittaria Trifolia,Litchi Chinensis,antidepressant effects, methanolic extract