Published April 1, 2024 | Version v1
Journal article Open

Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

  • 1. ROR icon University Children's Hospital Tübingen
  • 2. DFG Cluster of Excellence 2180 iFIT
  • 3. German Cancer Consortium (DKTK), partnerside Tübingen, a partnership between German Cancer Research Center (DKFZ) and University Hospital Tübingen
  • 4. German Cancer Consortium (DKTK), partner site Tübingen, a partnership between German Cancer Research Center (DKFZ) and University Hospital Tübingen
  • 1. University Children's Hospital Tübingen
  • 2. School of Business and Economics, Faculty of Economics and Social Sciences, University of Tübingen
  • 3. Institute of Pathology and Neuropathology, University Hospital Tübingen and Comprehensive Cancer Center
  • 4. Institute of Tissue Medicine and Pathology (ITMP), University of Bern

Description

This study provides a novel, comprehensive immunophenotyping antibody panel for multiplexed tissue imaging, encompassed by a validated analysis workflow. It serves as a blueprint for an ultra-deep spatial analysis of subtypes and cellular states of immune and tumor cells, allowing for precise and unprecedented deciphering of tumor (immune) microenvironments. 

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Additional details

Related works

Is metadata for
Dataset: 10.3389/fimmu.2024.1383932 (DOI)

Software

Programming language
R , Python console