Content-Aware Frame Interpolation Microscopy Datasets

Dataset 1: 1_BF_SHSY5Y_data.zip

Speed: Image frequency: 1 seconds per image.
Microscope: Leica SP8, 63x 1.40 NA oil objective

Image size: 512 x 512 x (time or z dimension varying depending on the depth of the stack or the duration of the recording)

File format: .tif (8-bit)

Cell Type: SH-SY5Y cells recorded on a confocal brightfield microscope.

Comments: Consisting of a mix of axial stacks and temporal sequences.

Dataset 2: 2_Flu_lyso_data.zip

Speed: Image frequency: 1 seconds per image.

Microscope: Leica SP8, 63x 1.40 NA oil objective

Image size: 512 x 512 x (time or z dimension varying depending on the depth of the stack or the duration of the recording)

File format: .tif (8-bit)

Cell Type: FLCS1  fluorescently labelled lysosomes in SHSY5Y cells recorded on point-scanning confocal microscope. (FLCS1:  fluorescent Copper(I) ligand which accumulates in lysosomes: see https://doi.org/10.1002/anie.202109349)

Comments: Consisting of a mix of axial stacks and temporal sequences.

Dataset 3: 3_D.Discoideum_Data.zip

Speed: Image frequency: 2-minutes per image. (was originally recorded as 3D+T dataset - here reassembled as 2D+T)

Microscope: inverted spinning disc confocal microscope (3i) using 63x 1.4NA oil objective and equipped with a prime95B CMOS camera (photometrics).
Image size: 512 x 512 (downsized from an original image size of 1200 x 1200 and augmented by zooming into the corners of the original image)

File format: .tif (8-bit)

Cell Type: GFP labelled Dictyostelium discoideum cells recorded on inverted spinning disc confocal microscope.

Dataset 4: 4_Synth_Data.zip

Speed: -

Microscope: -

Particle and Image properties: Switching Uniform; xy pixel size: 15; y borders simu: 50; extinction rate: 0.003; density: 40; SNR: 10; sequence length: 225; Sigma brownian: 1; q1: 2; vmin: 2; vmax: 12; Probe directed Brownian: 0.3; Probe Brownian directed: 0.3

Image size: 512 x 512

File format: .tif (8-bit)

Type: Simulated particles created with ICY ISBI particle tracking challenge 2015 plugin

Dataset 5: 5_CelegansEmbryo_alphaTub.zip

Speed: Image frequency: 50 ms per image.
Microscope: Lattice light sheet microscope

Image size: 512 x 512 x 300 x 49 (z-spacing 100 nm)

File format: .tif (8-bit)

Cell Type: C.Elegans embryo with GFP alpha tubulin labeling

Comments: -

Dataset 6: 6_Car-T_Data.zip

Speed: Image frequency: 50 ms per image.
Microscope: Lattice light sheet microscope

Image size: 512 x 512 x 150 x 49 (z-spacing 100 nm)

File format: .tif (8-bit)

Cell Type: CAR-T cells placed on a passivated 5 mm cover glass

Comments: -

Dataset 7: 7_MDCK_Data.zip

Speed: Image frequency: 50 ms per image.
Microscope: Lattice light sheet microscope

Image size: 512 x 512 x 58 x 100 (z-spacing 100 nm)

File format: .tif (8-bit)

Cell Type: MDCK cells

Comments: -

Dataset 8: 8_CelegansEmbryo_PHGFP_Data.zip

Speed: Image frequency: 50 ms per image.
Microscope: Lattice light sheet microscope

Image size: 512 x 512 x 300 x 301

File format: .tif (8-bit)

Cell Type: C. Elegans embryo with GFP-PH labeling

Comments: -

Source Data Files 1-3: 

Source_data_Actin_Fig_6_S9.zip

Source_data_BF_Fig_6_E8.zip

Source_Data_BF_Upscale_S29_S30.zip

Source_data_C_Elegans_alphaTub_Fig_S11_S12_S13.zip

Source_data_C_Elegans_GFP_Fig_S3_S20_S21_S22.zip

Source_data_DD_Fig6_E9.zip

Source_data_EM_Fig_S8.zip

Source_data_EM_stack_upscale_Fig_S33_S34.zip

Source_data_Fibronectin_3D_Supp_Video_S11.zip

Source_data_Fibronectin_z_Supp_Video_S11.zip

Source_data_Fibronectin-t_Fig_6_E10.zip

Source_Data_Fig_S7_E6.zip

Source_Data_Fig_E5.zip

Source_data_Lysosome_Fig_6_E7.zip

Source_data_Lysosomes_z-proj_Fig_5.zip

Source_Data_MDCK_Fig_S17_S18_S19.zip

Source_data_Mitochondria_Fig_2_S2.zip

Source_data_SynData_Fig_3.zip

Source_Data_SynData_multi_step_int_Fig_4.zip

Source_data_SynData_Tracking_Fig_3.zip

Source_Data_SynDataNoise_Fig_E6_S7.zip

Source_Data_Lysosome_zoom_Fig_S27_S28.zip

Source_Data_DD_zoom_Fig_S31_S32.zip

For reproducing figures

Comments: Within those zip-files folder names containing "DS" stands for down-samples (removing every other image from the sequence), "DAIN" stands for interpolation carried out by DAIN, "ZI" symbolizes interpolation carried out by ZS.