Quantification of sulfolane, a metabolite of busulfan in plasma by gas chromatography and tandem mass spectrometry: a new method for evaluating the role of sulfolane and metabolic fate of busulfan

We developed a novel method for determination of sulfolane along with busulfan in plasma of patients receiving busulfan

Method was validated with acceptable levels of trueness and precision

Sulfolane is detectable at 4 h after the end of 1st dose infusion, and higher levels were observed after the 9th dose infusion of busulfan

Understanding kinetics of sulfolane and understanding the role of sulfolane in clinical outcomes  and toxicity of busulfan treatment is possible with this method