PHYTOCHEMICAL EVALUATION, ANTIINFLAMATORY ACTIVITY, AND DETERMINATION OF BIOACTIVE COMPONENTS FROM LEAVES OF MUSSAENDA FRONDOSA

The present study is focused on phytochemical screening of aqueous and methanolic leaf extracts of Mussaenda frondosa (L) and anti-inflammatory activity of different leaves extracts of Mussaenda frondosa, by using carageenin indused paw edema method. The preliminary phytochemical investigation showed the presence of steroids and phenolic compounds, glycosides, carbohydrates etc.The compounds β-sitosterol, lupeol, mussendoside, mussendoside-M, sanshide-methyl ester, 3-palmitoyl lupeol have been isolated from different leaf extracts of Mussaenda frondosa(L). Further, these isolated structures were established by spectral analysis and direct comparison with authentic samples. This is the first report of occurrence of these compounds from Mussaenda frondosa(L).From the anti-inflammatory activity method i.e.carageenin indused paw edema method, the petroleum ether, ethyl acetate extracts were exhibited significance anti-inflammatory activity (P < 0.01). Due to presence of steroids and flavonoidal glycosides. Whereas butanol extract showed marked anti-inflammatory activity (P < 0.05) when compared standard. The results clearly indicate that ethyl acetate and petroleum ether extracts of Mussaenda frondosa are effective against inflammatory diseases.