EXPLORATION OF IN-VITRO ANTHELMINTIC ACTIVITY AND GCMS FINGERPRINT PROFILING OF TRADITIONAL SIDDHA FORMULATION CHITRAMUTTINEI

Helminthiasis becomes a global health issue in developing countries. They are the most common infectious agents of humans and produce a global burden of disease compared to malaria and tuberculosis. Commonly prescribed drug for clinical management of helminths infestation is Albendazole, but this drug exert some potential toxicity in the individuals upon usage.Hence there is a need foran alternative medicine in particular. Traditional medicinal system like Siddha are known for clinically effective against helminth infections and also devoid of adverse event. Siddha system of medicine become popular throughout the world in recent days as it claims high curative value with minimum toxicity and have less side effects. The present research work aimed at evaluating the in-vitro anthelmintic activity of the formulation ChitramuttiNei (CN) using Indian adult earthworm and also to investigate the bioactive phytocomponents present in the formulation using GCMS analysis. The result of GCMS analysis reveals the presence of most significant phytocomponents like Stigmasterol, Oleic acid, n-Hexadecanoic acid etc. The results of In-vitroanthelmintic activity of CN clearly shown that the maximum time taken for the test drug CN at the dose of 10gm to cause death of worms is about 303.3± 6.89mins, similarly the time taken of CN at the dose of 20gm would be 260.5 ± 20.16mins for standard drug albendazole it was 88± 7.30mins at the concentration of 100mg/ml. From the result of the study it was concluded that the test drug CN possesses convincing anthelmintic property and also have numerous bioactive phytocomponents with versatile biological action