We present a list of the anuran amphibians of the state of Espírito Santo, southeastern Brazil. The list was compiled from data gathered from fieldwork over the course of the past 20 years in different localities, and from literature records and voucher specimens deposited in scientific collections. Our list comprises 133 species. Patchy sampling efforts and recent species descriptions suggest that the list may increase significantly with further sampling, considering the presence of several geographical gaps. The need for adequate sampling in these gap areas is highlighted.