Role of TRP channels in dinoflagellate mechanotransduction

This repository contains several files associated with the publication:

Transcriptome: Lingulodinium polyedra transcriptome assembled from 100 bp paired-end Illumina RNA sequences available at http://www.ebi.ac.uk/ (run accession SRR1184657) using the assembly and annotation pipeline “MakeMyTranscriptome” (https://github.com/bluegenes/MakeMyTranscriptome), which leverages the Trinity de novo assembler (v2.1.1; Grabherr 2011). The transcriptome contains 183,451 transcripts, with an N50 of 1161, and 66.5% GC (guanine-cytosine) content. Annotation-based assessment using BUSCO showed that this transcriptome contained 62% of eukaryotic genes predicted to be present in all eukaryotic assemblies.  Open reading frames (ORFs) predicted using transDecoder are also provided as a second file (ends with .pep).

TRP-like polypeptide sequences: Transient Potential Receptor (TRP)-like polypeptide sequences identified in transcriptomic data from the dinoflagellate Lingulodinium polyedra.

Video: Video of luminescence from the dinoflagellate Lingulodinium polyedra in response to capsaicin treatment. Samples consisted of 1 ml volumes of L. polyedra culture containing approximately 7000 cells, kept in two separate glass vials. The injection of 0.1 ml volumes of control solution (left vial) and capsaicin (30 µM final concentration, right vial) at a rate of 1ml min^-1 caused some light response due to stimulation of cells by the fluid addition. However, following fluid addition the capsaicin treatment clearly stimulated luminescence in the L. polyedra cells, seen as individual sources of light, while the cells in the control treatment produced essentially no light.