Journal article Open Access

Pregnenolone separation from cholesterol using Sephadex LH-20 mini-columns

Hanukoglu, Israel; Jefcoate, Colin R.

This article reports a new procedure for the separation of radiolabeled pregnenolone and cholesterol using minicolumns of Sephadex LH-20 and a simple solvent system. Additionally, we describe a Sephadex LH-20 procedure for the purification of radiolabeled cholesterol.
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