Journal article Open Access

cDNA sequence of adrenodoxin reductase. Identification of NADP-binding sites in oxidoreductases

Hanukoglu, Israel; Gutfinger, Tamar

Adrenodoxin reductase is an NADP dependent flavoenzyme which functions as the reductase of mitochondrial P 450 systems. We sequenced two adrenodoxin reductase cDNAs isolated from a bovine adrenal cortex cDNA library. The deduced amino acid sequence shows no similarity to the sequence of the microsomal P 450 systems or other known protein sequences. Nonetheless, by sequence analysis and c comparisons with known sequences of dinucleotide-binding folds of two NADP-binding flavoenzymes, two regions of adrenodoxin reductase sequence were identified as the FAD- and NADP-binding sites. These analyses revealed a consensus sequence for the NADP-binding dinucleotide fold (GXGXXAXXXAXXXXXXG, in one-letter amino acid code) that differs from FAD and NAD-binding dinucleotide-fold sequences. In the data base of protein sequences, the NADP-binding-site sequence appears solely in NADP-dependent enzymes, the binding sites of which were not known to date. Thus, this sequence may be used for identification of a certain type of NADP-binding site of enzymes that show no significant sequence similarity.

Files (593.0 kB)
Name Size
article.pdf
md5:ef5f65f77c4710db90819fc98c135a57
593.0 kB Download
2
1
views
downloads
Views 2
Downloads 1
Data volume 593.0 kB
Unique views 2
Unique downloads 1

Share

Cite as