Coupling liquid chromatography to Orbitrap mass spectrometry

Abstract 48 The Orbitrap mass analyzer has become a mainstream mass spectrometry technique. In addition 49 to providing a brief introduction to the Orbitrap technology and its continuing development, this 50 article reviews the most recent publications quoting the use of the Orbitrap detection for a variety 51 of chromatographic separation techniques. Its coupling to reversed-phase liquid chromatography 52 (LC) represents undoubtedly the most ubiquitous approach to both small molecule and proteomic 53 analyses. Multidimensional LC separations have an important role to play in the proteomics 54 applications while an ultra-high-pressure LC is more frequently encountered in the area of 55 metabolomics and metabolite analysis. Recently, special chromatographic techniques such as 56 hydrophilic interaction chromatography and its variations have also been also cited with the 57 Orbitrap detection.