Published March 19, 2013 | Version v1
Journal article Open

Potential applications for Annona squamosa leaf extract in the treatment and prevention of foodborne bacterial disease

Authors/Creators

  • 1. Faculty of Technology, Mahasarakham University, Khamriang, Kantarawichai, Maha Sarakham 44150, Thailand
  • 2. Department of Chemistry, Faculty of Science, Center of Excellence for Innovation in Chemistry (PERCH-CIC), Mahasarakham University, Khamriang, Kantarawichai, Maha Sarakham 44150, Thailand
  • 3. Faculty of Medicine, Mahasarakham University, Khamriang, Kantarawichai, Maha Sarakham 44150, Thailand

Description

Foodborne disease is a major public health problem.  The present study examined Annona squamosa leaves, which are traditionally used to treat diarrhea and other infections, for their potential to be used in modern food safety or medicine.  Active constituents were partially purified by ethanol extraction and column chromatography.  MICs of the extract were 62.5 to 125 μg/mL against Bacillus cereus, Listeria monocytogenes and Staphylococcus aureus, and 250 μg/mL against Campylobacter jejuni.  In time-kill assays, 500 μg/mL of the extract reduced colony forming unit numbers of C. jejuni almost 10 000-fold within 12 hours.  Similar decreases were seen against B. cereus, but over a longer time-frame.  LC-MS analysis indicated the presence of reticuline and oxophoebine.  Assessment of stability by MIC assay showed activity was heat-labile, with loss of activity greatest following high temperature treatments.  Activity was relatively stable at refrigeration temperature.  These results indicate A. squamosa has broad-spectrum but heat-labile activity against foodborne bacterial pathogens, and bactericidal activity against B. cereus and C. jejuni.  This bactericidal activity is not sufficiently rapid for A. squamosa to be used as a food sanitizer, but the extract could potentially be developed as an additive for refrigerated foods, or a modern treatment for foodborne illness.  

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References

  • World Health Organization. (2007) Food Saftey and Food-borne Illness (Fact Sheet No. 237). WHO, Geneva.
  • Centers for Disease Control and Prevention. (2011) Vital signs: incidence and trends of infection with pathogens transmitted commonly through food- Foodborne Diseases Active Surveillance Network, 10 U.S. sites, 1996-2010. Morbidity and Mortality Weekly Report, 60, 749-755.
  • United States Department of Agriculture. (2011) Foodborne Illness and Disease. In Foodborne Illness: What Consumers Need to Know. USDA, Washington DC.
  • Collignon P, Powers JH, Chiller TM, Aidara-Kane A, Aarestrup FM. (2009) World Health Organization ranking of antimicrobials according to their importance in human medicine: a critical step for developing risk management strategies for the use of antimicrobials in food production animals. Clinical Infectious Diseases, 49, 132-141.
  • Casani S, Rouhany M, Knøchel S. (2005) A discussion paper on challenges and limitations to water reuse and hygiene in the food industry. Water Research, 39, 1134-1146.
  • Zengin N, Yüzbaşioğlu D, Unal F, Yilmaz S, Aksoy H. (2011) The evaluation of the genotoxicity of two food preservatives: sodium benzoate and potassium benzoate. Food and Chemical Toxicology, 49, 763-769.
  • Luangtongkum T, Jeon B, Han J, Plummer P, Logue CM, Zhang QJ. (2009) Antibiotic resistance in Campylobacter: emergence, transmission and persistence. Future Microbiology, 4, 189-200.
  • Tajkarimi MM, Ibrahim SA, Cliver DO. (2010) Antimicrobial herb and spice compounds in food. Food Control, 21, 1199-1218.
  • Ríos JL, Recio MC. (2005) Medicinal plants and antimicrobial activity. Journal of Ethnopharmacology, 100, 80-84.
  • Ceylan E, Fung DYC. (2004) Antimicrobial activity of spices. Journal of Rapid Methods and Automation in Microbiology, 12, 1-55.
  • Chrystal EJT, Wrigley SK, Thomas R, Nicholson N. (2000) Biodiversity: New Leads for the Pharmaceutical and Agrochemical Industries. Royal Society of Chemistry, Cambridge. p. 205-213.
  • Silván JM, Mingo E, Hidalgo M, de Pascual-Teresa S, Carrascosa AV, Martinez-Rodriguez AJ. (2013) Antibacterial activity of a grape seed extract and its fractions against Campylobacter spp. Food Control, 29, 25-31.
  • Kim SY, Kang DH, Kim JK, Ha YG, Hwang JY, Kim T, Lee SH. (2011) Antimicrobial activity of plant extracts against Salmonella Typhimurium, Escherichia coli O157:H7, and Listeria monocytogenes on fresh lettuce. Journal of Food Science, 76, M41-M46.
  • Yusha'u M, Taura DW, Bello B, Abdullahi N. (2011) Screening of Annona squamosa extracts for antibacterial activity against some respiratory tract isolates. International Research of Pharmacy and Pharmacology, 1, 237-241.
  • Park YJ, Chen J. (2011) Inactivation of shiga toxin-producing Escherichia coli (STEC) and degradation and removal of cellulose from STEC surfaces by using selected enzymatic and chemical treatments. Applied and Environmental Microbiology, 77, 8532-8537.
  • Centers for Disease Control and Prevention. (2004) National Antimicrobial Resistance Monitoring System for Enteric Bacteria (NARMS): Human Isolates Final Report, 2002. CDC, Atlanta GA.
  • Rohwer J, Green P, Bittrich V, Götz A, Kramer K, Kubitzki K, Huber H, Bayer C, Kadereit J, Jeffrey C. (1990) The Families and Genera of Vascular Plants. Springer, Berlin.
  • Orhan I, Özcelik B, Şener B. (2007) Antiviral and antimicrobial evaluation of some heterocyclic compounds from Turkish plants. In Bioactive Heterocycles V, Khan M. (Ed). Springer, Berlin. p. 303-323.
  • Drewnowski A, Gomez-Carneros C. (2000 ) Bitter taste, phytonutrients, and the consumer: a review The American Journal of Clinical Nutrition 72 1424-1435
  • Clinical and Laboratory Standards Institute. (1999) Methods for determining bactericidal activity of antimicrobial agents; Approved guideline (M26-A). CLSI, Wayne PA.
  • Suarez M, Haenni M, Canarelli S, Fisch F, Chodanowski P, Servis C, Michielin O, Freitag R, Moreillon P, Mermod N. (2005) Structure-function characterization and optimization of a plant-derived antibacterial peptide. Antimicrobial Agents and Chemotherapy, 49, 3847-3857.
  • Cushnie TPT, Taylor PW, Nagaoka Y, Uesato S, Hara Y, Lamb AJ. (2008) Investigation of the antibacterial activity of 3-O-octanoyl-(-)-epicatechin. Journal of Applied Microbiology, 105, 1461-1469.
  • Sakoulas G, Eliopouios GM, Fowler VG, Moellering RC, Novick RP, Lucindo N, Yeaman MR, Bayer AS. (2005) Reduced susceptibility of Staphylococcus aureus to vancomycin and platelet microbicidal protein correlates with defective autolysis and loss of accessory gene regulator (agr) function. Antimicrobial Agents and Chemotherapy, 49, 2687-2692.
  • Naznin MT, Akagawa M, Okukawa K, Maeda T, Morita N. (2008) Characterization of E- and Z-ajoene obtained from different varieties of garlics. Food Chemistry, 106, 1113-1119.
  • Noriega E, Newman J, Saggers E, Robertson J, Laca A, Díaz M, Brocklehurst TF. (2010) Antilisterial activity of carrots: effect of temperature and properties of different carrot fractions. Food Research International, 43, 2425-2431.
  • Dholvitayakhun A, Cushnie TPT, Trachoo N. (2012) Antibacterial activity of three medicinal Thai plants against Campylobacter jejuni and other foodborne pathogens. Natural Product Research, 26, 356-363.
  • Ratnam KV, Raju RRV. (2008) In vitro antimicrobial screening of the fruit extracts of two Syzygium species (Myrtaceae). Advances in Biological Research, 2, 17-20.
  • Guinaudeau H, Lebœuf M, Cavé A. (1994) Aporphinoid alkaloids, V. Journal of Natural Products, 57, 1033-1135.
  • Kotake Y, Okuda K, Kamizono M, Matsumoto N, Tanahashi T, Hara H, Caparros-Lefebvre D, Ohta S. (2004) Detection and determination of reticuline and N-methylcoculaurine in the Annonaceae family using liquid chromatography-tandem mass spectrometry. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 806, 75-78.